Journal: Mucosal Immunology

Article Title: Microbial regulation of intestinal motility provides resistance against helminth infection

doi: 10.1038/s41385-022-00498-8

Figure Lengend Snippet: a – f SPF and antibiotic-treated mice (enrofloxacin for 2 weeks then amoxicillin and clavulanic acid for the rest of the experiment) were infected for 0 to 21 days with 200 Hpb larvae. Intestine were processed for histological staining of ( a ) DCLK1 (tuft cells (*), green staining) ( c ) mucus with PAS (goblet cells, purple staining) or ( e ) RELM-β (goblet cells, brown staining). All scales represent 200 μm. For each staining, representative images of day 0 and 14 post infected are displayed. Quantification of ( b ) DCLK1 + , ( d ) mucus-producing and ( f ) RELM-β + cells are represented as an average of at least five fields of view for each individual. Data were pooled from two independent experiments with one experiment including day 0 and 14 time points and the 2nd experiment including the day 28 time point. Each dot represents and individual animal. Data were analysed using two-way ANOVA. b The number of mice per time point are: (1) for SPF mice D0 = 4, D14 = 3 and D28 = 4; (2) for antibiotic-treated mice D0 = 3, D14 = 4 and D28 = 5. d The number of mice per time point are: (1) for SPF mice D0 = 3, D14 = 4 and D28 = 5; (2) For antibiotic-treated mice D0 = 4, D14 = 4 and D28 = 5. f The number of mice per time point are: (1) for SPF mice D0 = 4, D14 = 3 and D28 = 4; (2) for antibiotic-treated mice D0 = 3, D14 = 4 and D28 = 5. * display statically significant differences between groups and # display statistically significant differences compared to the naïve group (0 days post-infection).

Article Snippet: Sections were stained with primary antibodies, anti-RELM-β antibody (Peprotech 500-P215) or control IgG, followed by incubation with secondary antibody, anti-rabbit IgG biotinylated (Jackson Immunoresearch 711-065-152).

Techniques: Infection, Staining